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为了探讨7Li离子束处理对植物种子的诱变效应,以冬小麦品种新麦18和轮选987的干种子为试验材料,研究了7Li离子束处理对小麦幼苗生长和M1代基因组DNA变异的影响。结果表明,7Li离子束处理虽然对小麦干种子的发芽率和幼苗根的生长无显著影响,但对幼苗高度影响极显著,而且20 Gy剂量的7Li离子束就可使幼苗高降低到对照的一半左右。SSR分析表明,7Li离子束可明显诱发小麦M1代基因组DNA SSR带型的变化。两个品种各处理间的平均多态性频率都达到了13.1%,且多态性片断的类型以扩增片断的增加和缺失为主。以上结果表明,7Li离子束处理小麦种子具有较高的生物损伤效应,可以造成DNA的变异,从而有可能产生可遗传的变异。
In order to investigate the mutagenic effects of 7Li ion beam on plant seeds, the effects of 7Li ion beam treatment on the growth of wheat seedlings and the variation of genomic DNA of M1 generation were studied using the dry seeds of winter wheat variety Xinmai 18 and wheats 987 as experimental materials. The results showed that although the 7Li ion beam treatment had no significant effect on the germination rate of wheat seeds and the root growth of the seedlings, the seedling height was significantly affected by the 7Li ion beam treatment, and the seedling height was reduced to half by the 7Li ion beam of 20 Gy about. SSR analysis showed that 7Li ion beam could obviously induce the change of genotypes of wheat M1 genomic DNA SSR. The average polymorphism frequency of the two cultivars reached 13.1%, and the polymorphic fragments were mainly amplified and deleted. The above results show that 7Li ion beam treatment of wheat seeds has a high biological damage effect, can cause DNA variation, which may have heritable mutations.