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目的比较不同类型干细胞分泌细胞因子的水平,探讨其潜在的临床意义。方法建立人类胚胎干细胞(hESCs)、脐带间充质干细胞(UMSCs)、羊膜间充质干细胞(AMSCs)株系;实时荧光定量聚合酶链反应(RT-qPCR)分析干细胞分泌细胞因子水平:(1)促炎因子,包括白细胞介素-6(IL-6)、白细胞介素-8(IL-8)和肿瘤坏死因子(TNF-α);(2)抑炎因子白细胞介素-10(IL-10);(3)生长因子,包括肝细胞生长因子(HGF)、脑源性神经营养因子(BDNF)、干细胞因子(SCF)和血管内皮生长因子(VEGF);(4)基质金属蛋白酶MMP-1及其抑制剂TIMP-1;(5)血管形成因子(Angiogenin),白血病抑制因子(LIF)以及骨形成蛋白(BMP4)。结果女性UMSCs分泌生长因子、血管生成因子、造血因子、抑炎因子等的能力显著强于hESCs、AMSCs(P<0.05);女性hESCs、男性UMSCs分泌金属蛋白酶的水平显著高于其它干细胞(P<0.05)。结论女性UMSCs可能具有更好的促进细胞生长、改善血供的能力;而女性hESCs、男性UMSCs则可能具有更强的降解结缔组织的能力。
Objective To compare the levels of cytokines secreted by different types of stem cells and to explore its potential clinical significance. Methods Human embryonic stem cells (hESCs), umbilical cord mesenchymal stem cells (UMSCs) and amniotic mesenchymal stem cells (AMSCs) were established. The levels of cytokines secreted by stem cells were analyzed by real-time fluorescent quantitative polymerase chain reaction ) Proinflammatory cytokines including interleukin-6 (IL-6), interleukin-8 (IL-8) and tumor necrosis factor (TNF- (3) growth factors including hepatocyte growth factor (HGF), brain derived neurotrophic factor (BDNF), stem cell factor (SCF) and vascular endothelial growth factor (VEGF); (4) matrix metalloproteinase MMP -1 and its inhibitor TIMP-1; (5) Angiogenin, leukemia inhibitory factor (LIF) and bone morphogenetic protein (BMP4). Results The ability of female UMSCs to secrete growth factors, angiogenic factors, hematopoietic factors and anti-inflammatory factors was significantly stronger than that of hESCs and AMSCs (P <0.05). The levels of metalloproteinases secreted by female hESCs and male UMSCs were significantly higher than those of other stem cells (P < 0.05). Conclusion Female UMSCs may have better ability of promoting cell growth and blood supply, while female hESCs and male UMSCs may have more ability to degrade connective tissue.