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探讨肾母细胞瘤过度表达基因NOV/CCN3表达产物在肝病患者外周血中的水平及其在肝癌演进中的病理意义。采用ELISA方法共检测380例原发性肝细胞癌(HCC)患者,92例肝硬化(LC)患者和102例正常人血清中NOV蛋白表达水平,分析其表达与肝癌的临床病理学特征的关系。LC组NOV含量高于HCC组及正常对照组(P<0.001)。将LC组与正常对照组比较,以血清NOV水平做ROC曲线,曲线下面积为0.741,NOV在cutoff值为7.812ng/ml时敏感度为67.4%,特异度为73.5%。在HCC组中,NOV水平与肿瘤的TNM分期、肿瘤大小呈负相关。NOV对于AFP阴性人群的诊断效率经ROC曲线分析提示,曲线下面积为0.803,NOV在cutoff值为7.68ng/ml时,敏感度为83.8%,特异度为71.6%。NOV可辅助诊断肝硬化、肝癌,该分子可能与肝硬化发生相关并在肝癌的演进中发挥负性调控作用。
To investigate the expression of NOV / CCN3 overexpression gene in nephroblastoma in peripheral blood of patients with liver disease and its pathological significance in the progression of liver cancer. The serum levels of NOV in 380 cases of primary hepatocellular carcinoma (HCC), 92 cases of cirrhosis (LC) and 102 normal persons were detected by ELISA. The relationship between the expression of NOV and the clinicopathological features of hepatocellular carcinoma . The NO level in LC group was higher than that in HCC group and normal control group (P <0.001). The ROC curve of serum NOV level was compared between LC group and normal control group. The area under the curve was 0.741. The NOV of the LCV group was 67.4% and the specificity was 73.5% when the cutoff value was 7.812 ng / ml. In HCC group, NOV level was negatively correlated with tumor TNM stage and tumor size. The diagnostic efficiency of NOV for AFP negative population by ROC curve analysis showed that the area under the curve was 0.803, the NOV was 83.8% and the specificity was 71.6% when the cutoff was 7.68ng / ml. NOV can help diagnose cirrhosis and liver cancer, which may be related to the occurrence of cirrhosis and play a negative regulatory role in the development of liver cancer.