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研究Folin-Ciocalteu比色法测定石榴皮提取物中多酚含量的优化条件,并对该方法的稳定性、精密度、重现性、回收率进行了实验。结果表明,石榴皮提取物中多酚含量测定的最佳条件为:Folin-Ciocalteu试剂1.00mL、10.0g/100mL Na2CO33.00mL、反应温度25℃、反应时间120min、测定波长672nm,所用没食子酸标准品在10—100μg/mL范围内与吸光度呈良好的线性关系(y=0.0098x+0.0148,R2=0.9982)。该法简便、快速、准确,是检测石榴皮提取物中总多酚的可靠方法。
The optimal conditions for the determination of polyphenol content in pomegranate peel extract by Folin-Ciocalteu colorimetry were studied. The stability, precision, reproducibility and recovery of the method were also tested. The results showed that the optimal conditions for the determination of polyphenol content in pomegranate peel extracts were: Folin-Ciocalteu reagent 1.00mL, 10.0g / 100mL Na2CO3 33.00mL, reaction temperature 25 ℃, reaction time 120min, determination wavelength 672nm, The product showed a good linear relationship with absorbance in the range of 10-100μg / mL (y = 0.0098x + 0.0148, R2 = 0.9982). The method is simple, rapid and accurate and is a reliable method for detecting total polyphenols in pomegranate peel extract.