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精子与卵母细胞的结合是哺乳动物受精过程中决定性的步骤。配子间的相互作用与精子浆膜表面特异性受体和卵子透明带(ZP)糖蛋白有关。 本研究是一项体外试验,应用促性腺激素释放激素(GnRH)拮抗剂抑制精子与透明带的结合。将活动的精子细胞(10~7个/ml)置于37℃下、5％CO_2大气、改良的Tyrode培养基中孵化。部分精子经盐水处理(对照组),另部分经不同温度的GnRH拮抗剂处理(实验组),然后每份精子行半透明带结合试验
Sperm and oocyte combination is a decisive step in mammalian fertilization. The interaction between gametes is related to the sperm serosal surface-specific receptors and the zona pellucida (ZP) glycoprotein. This study is an in vitro experiment that inhibits the binding of sperm to zona pellucida using a gonadotropin-releasing hormone (GnRH) antagonist. Active sperm cells (10-7 cells / ml) were incubated at 37 ° C in a 5% CO 2 atmosphere in a modified Tyrode medium. Some of the sperm were treated with saline (control group) and the other group were treated with GnRH antagonist (experimental group) at different temperatures. Then, each spermatozoon was treated with translucent band