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目的 肠道是新生儿窒息后受损最严重的器官之一。但目前国内外尚无一经典模型用于窒息后 肠道损伤的研究。本研究采用可逆性宫内缺血制作了大鼠窒息后肠道损伤模型。方法 钳夹足月孕鼠(E21)一 侧子宫和卵巢动静脉20min,以结扎侧的胎鼠为窒息组,对侧胎鼠为对照组。剖宫产取出胎鼠,复苏,代乳鼠代乳。 分别饲养0,24,48,72h后处死(每组每时点18只),取出肠组织行病理观察,评估肠黏膜损害指数(IMDI)。结果 对照鼠娩出后表现正常;缺血鼠娩出后出现皮肤青紫、呼吸减弱、四肢活动减少等窒息改变。缺血鼠病理学改变在 缺血后48h最重,IMDI明显上升(3.40±0.16),对照组肠粘膜几乎没有损害(0.00±0.00)。缺血后72h肠道损 伤明显恢复,IMDI降至0.60±0.21。结论 钳夹足月孕鼠一侧子宫、卵巢动静脉可以制成窒息后肠道损伤的动 物模型。
The intestine is one of the most severely damaged organs of a newborn after asphyxiation. However, at present, there is not a classical model for the study of intestinal injury after asphyxia. In this study, a reversible intrauterine ischemia model of rat intestinal injury after asphyxia was made. Methods The uterus and ovarian arteriovenous vein of one month pregnant rat (E21) were clamped for 20 minutes. The fetus on the ligation side was used as the asphyxia group and the contralateral fetus was used as the control group. Cesarean section fetus removed, recovery, on behalf of the milk rat milk. The animals were sacrificed at 0, 24, 48, and 72 h after sacrifice (18 animals per group at each time point). The intestinal tissues were removed for pathological observation to assess the intestinal mucosal damage index (IMDI). Results The control mice showed normal performance after delivery; the mice with ischemic mice showed bruising, decreased respiration and decreased suffocation such as decreased limb activity. The pathological changes of ischemic mice were the highest at 48h after ischemia, IMDI increased significantly (3.40 ± 0.16), while there was almost no damage in the control group (0.00 ± 0.00). At 72 hours after ischemia, the intestinal injury recovered obviously, IMDI dropped to 0.60 ± 0.21. Conclusion The uterus can be made into the animal model of intestinal injury after asphyxia by clamping the uterus on one side of full term pregnant rats.