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目的:探讨凋亡素2配体(Apo-2L)对放射线诱导肺癌95-D细胞凋亡的影响。方法:MTT法检测不同浓度凋亡素2配体在体外对肺癌95-D细胞的抑制率,将细胞分为4组,对照组、凋亡素2配体组、单纯照射组、凋亡素2配体+放射照射组,流式细胞仪检测各组凋亡率及细胞周期。结果:凋亡素2配体对95-D细胞的体外抑制作用明显,随着药物浓度的增大及时间的延长,抑制率明显增高(P<0.05)。流式细胞术显示凋亡素2配体与放射线联用能够使95-D细胞的凋亡率提高,与单用凋亡素2配体组及单纯放疗组相比,凋亡率差异显著(P<0.05)。结论:凋亡素2配体在体外具有抑制95-D细胞增殖的作用并能够促进细胞的凋亡,同时凋亡素2配体联合放射线可以明显提高肺癌95-D细胞的凋亡率。
Objective: To investigate the effect of apoptin 2 ligand (Apo-2L) on the apoptosis of 95-D cells induced by radiation. Methods: MTT assay was used to detect the inhibitory rates of different concentrations of apoptin 2 ligand on 95-D lung cancer cells in vitro. The cells were divided into 4 groups: control group, apoptin 2 ligand group, 2 ligand + radiation group, and the apoptosis rate and cell cycle of each group were detected by flow cytometry. Results: The inhibitory effect of apoptin 2 ligand on 95-D cells in vitro was obvious. With the increase of drug concentration and the prolongation of time, the inhibitory rate was significantly increased (P <0.05). Flow cytometry showed that the apoptosis rate of 95-D cells was enhanced by the combination of apolipoprotein 2 ligand and radiation, and the apoptosis rate was significantly different from that of the single apoptin 2 ligand group and radiotherapy alone group ( P <0.05). CONCLUSION: Apoptosis-inducing factor 2 ligand can inhibit the proliferation of 95-D cells in vitro and promote the apoptosis of cells. At the same time, the combination of apoptin 2 ligand and radiation can significantly increase the apoptosis rate of 95-D cells.