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在国内、外首次选用金黄色葡萄球菌(Staphylococc aureus)的No.180菌株用于病毒细菌协同凝集试验(Virobacterial agglutination test简称VBA)检测了来自马铃薯的4种不同形态粒子的病毒:马铃薯X病毒(PVX)、马铃薯丫病毒(PVY)、烟草花叶病毒(TMV)和马铃薯卷叶病毒(PLRV),其灵敏度达2.7～6.1ng/ml,检出病汁液的最大稀释度达10~4～10~5(PLRV1:500),较国内、外采用的Cown I菌株的灵敏度提高5～10倍。与血清学方法相比,VBA在2～3分种内就可获得结果,无假阳性反应,其灵敏度显著高于间接酶联法和免疫电镜,而接近于A蛋白酶联法。经抗血清致敏的菌体在4℃下保存4个月,对VBA检测的灵敏度没有影响。用VBA对采自田间的93个马铃薯病样进行检测,它们大多受2～3种病毒(PVX、PVY及PLRV)的复合侵染;和用间接酶联法及鉴别寄主检测的结果趋向一致。室内和田间试验均表明VBA灵敏度高、特异性强、快速简便和经济,尤其适合在基层单位中推广应用。
The No.180 strain of Staphylococcus aureus was selected for domestic use for the first time in the virus and bacteria agglutination test (VBA) to test the virus of four different morphological particles from potato: potato X virus PVX, PVY, TMV and PLRV, the sensitivity was 2.7 ~ 6.1ng / ml, and the maximum dilution of diseased juice was 10 ~ 4 ~ 10 ~ 5 (PLRV1: 500), compared with domestic and foreign Cown I strain increased by 5 to 10 times. Compared with serological methods, VBA obtained results within 2 to 3 minutes without any false positive reaction, and its sensitivity was significantly higher than that of indirect enzyme-linked immunosorbent assay (ELISA) and immunoelectron microscopy. Antisera-sensitized cells were stored at 4 ° C for 4 months and had no effect on the sensitivity of the VBA assay. Ninety-three potato samples collected from the field were detected by VBA. Most of them were infected by 2 or 3 viruses (PVX, PVY and PLRV). The results of indirect enzyme-linked immunosorbent assay and host-host detection tended to be consistent. Both indoor and field tests show that VBA is highly sensitive, specific, fast, easy and economical, and is especially suitable for general use in grass-roots units.