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探讨牛磺酸(taurine,Tau)通过p-p38通路对牛肺主动脉内皮细胞(PAECs)中ICAM-1,VCAM-1的影响及其作用机制。原代培养PAECs取4~12代细胞用于实验。分为5组:对照(control)组、缺氧(hyp)组、抑制剂(SB203580)组、给药(Tau)组、抑制剂+给药(SB+Tau)组,Tau的给药浓度为100 mmol·L~(-1),p38抑制剂SB203580浓度为20μmol·L~(-1),给药时间为12h。MTT检测不同浓度Tau对PAECs的抑制。使用Western blot及Real-time PCR方法检测p38通路蛋白及炎症因子ICAM-1,VCAM-1的表达情况。使用免疫荧光检测p38核位移情况。MTT结果显示随着Tau浓度增加,对PAECs增殖抑制增强。Western blot和Real-time PCR结果显示在蛋白水平及基因水平Tau都抑制ICAM-1,VCAM-1的表达。Western blot的结果和免疫荧光的结果均显示Tau可以抑制p38蛋白的活化。Tau可能通过p38 MAPK通路抑制由缺氧引起的牛肺主动脉内皮细胞中ICAM-1,VCAM-1的表达。
To investigate the effect of taurine (Tau) on ICAM-1, VCAM-1 in bovine pulmonary aortic endothelial cells (PAECs) through p-p38 pathway and its mechanism. Primary culture of PAECs from 4 to 12 generations of cells used in the experiment. The rats in control group, hypoxia group, SB203580 group, Tau group and SB + Tau group were divided into 5 groups: Tau group 100 mmol·L -1, p38 inhibitor SB203580 was 20 μmol·L -1, and the administration time was 12 h. Inhibition of PAECs by MTT assay with different concentrations of Tau. Western blot and Real-time PCR were used to detect the expression of p38 pathway protein and ICAM-1 and VCAM-1. Immunofluorescence was used to detect p38 nuclear shift. The results of MTT showed that with the increase of Tau concentration, the proliferation inhibition of PAECs was enhanced. Western blot and Real-time PCR results showed that ICAM-1 and VCAM-1 expression were inhibited both at the protein level and at the gene level. Western blot results and immunofluorescence results show that Tau can inhibit p38 protein activation. Tau may inhibit the expression of ICAM-1, VCAM-1 in bovine aortic endothelial cells induced by hypoxia through the p38 MAPK pathway.