论文部分内容阅读
目的 :分析T细胞 -急性淋巴细胞白血病 (T -ALL)病人的T细胞克隆性。方法 :利用RT -PCR方法分析 6例T -ALL和 10例正常人外周血单个核细胞中 2 4个T细胞受体变异 β(TCRVβ)基因的CDR3长度 ,PCR产物再进一步进行基因扫描和序列分析。结果 :3例病人的某些TCRVβ亚家族T细胞呈单克隆或寡克隆性增殖 ,主要为Vβ2、3、6、9、2 1和 2 4。其它 3例及正常人均表现为多克隆性增殖T细胞。 结论 :部分T -ALL来自于TCRVβ亚家族克隆性增殖T细胞。该方法有助于临床上检测微小残留病变
Purpose : To analyze the clonality of T cells in T-cell patients with acute lymphoblastic leukemia (T-ALL). Methods: RT-PCR was used to analyze the CDR3 length of 24 T cell receptor variants (TCRVβ) genes in peripheral blood mononuclear cells from 6 patients with T-ALL and 10 normal controls. PCR products were further used for gene scanning and sequence analysis. analysis. RESULTS: The TCR Vβ subfamily T cells of 3 patients showed monoclonal or oligoclonal proliferation, mainly Vβ2, 3, 6, 9, 21, and 24. The other 3 cases and normal individuals showed polyclonal proliferating T cells. Conclusion: Part of T-ALL is derived from TCRV[beta] subfamily cloned proliferating T cells. This method is helpful for clinical detection of minimal residual disease