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目的通过氯化锂-匹罗卡品(Li-PILO)诱导的癫癎大鼠模型,观察左乙拉西坦(LEV)对大鼠海马组织中神经细胞黏附分子(NCAM)和生长相关蛋白-43(GAP-43)mRNA的表达变化,为阐明LEV的抗癫癎机制及其剂量效应提供实验依据。方法将48只Wistar大鼠随机分成对照组、Li-PILO组(模型组)、150 mg/kg LEV组和300 mg/kg LEV组,每组12只。对LEV组大鼠予以LEV灌胃,首次灌胃时间为癫癎持续状态后6 h,剂量分别为150和300 mg/kg,每日一次,持续2周。Real-time PCR法测定各组大鼠海马组织中NCAM和GAP-43的mRNA表达。结果模型组大鼠海马NCAM及GAP-43的mRNA表达水平明显高于正常对照组(P<0.05),150 mg/kg LEV组和300 mg/kg LEV组明显低于模型组(P<0.05),其中300 mg/kg LEV组明显低于150 mg/kg LEV组(P<0.05)。结论 Li-PILO诱导癫癎大鼠海马NCAM和GAP-43 mRNA表达上调,LEV能够抑制NCAM和GAP-43 mRNA的表达,其抑制效果与剂量有关。
Objective To investigate the effects of levetiracetam (LEV) on the expression of neural cell adhesion molecule (NCAM) and growth-associated protein-1 in hippocampus of rats induced by lithium-pilocarpine 43 (GAP-43) mRNA expression changes in order to elucidate the anti-epileptic mechanism of LEV and its dose-response provide experimental evidence. Methods 48 Wistar rats were randomly divided into control group, Li-PILO group (model group), 150 mg / kg LEV group and 300 mg / kg LEV group, with 12 rats in each group. LEV group rats were given LEV intragastric administration, the first intragastric administration of 6 h after epilepticus sustained state, the dose was 150 and 300 mg / kg, once daily for 2 weeks. Real-time PCR was used to detect the mRNA expression of NCAM and GAP-43 in hippocampus of rats in each group. Results The mRNA expression levels of NCAM and GAP-43 in the hippocampus of the model group were significantly higher than those in the normal control group (P <0.05). The levels of NCAM and GAP-43 mRNA in the hippocampus of the 150 mg / kg LEV group and 300 mg / kg LEV group were significantly lower than those of the model group , Of which 300 mg / kg LEV group was significantly lower than 150 mg / kg LEV group (P <0.05). Conclusions Li-PILO induces up-regulation of NCAM and GAP-43 mRNA expression in the hippocampus of epileptic rats. LEV can inhibit the expression of NCAM and GAP-43 mRNA, and its inhibitory effect is dose-dependent.