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目的:探究双酚A对青春期前雌性大鼠卵泡发育相关基因表达的影响。方法:将21日龄Wistar大鼠分成H、M、L和C组,H、M、L组分别予160、40、10 mg·kg~(-1)的双酚A腹腔注射,C组予相同容量的精制橄榄油。1周提取卵巢组织进行定量PCR检测和蛋白印迹分析,比较各组卵巢组织中Kitlg、Figla、H1foo、AMH基因mRNA和蛋白的表达情况。结果:H组大鼠卵巢Kitlg和Fitla mRNA表达量低于C、L、M组(P<0.05),M、L、C组间差异无统计学意义(P>0.05);与C组比较,其它3组大鼠卵巢H1foo mRNA表达量都显著降低(P<0.05),L、M、H组间差异无统计学意义(P>0.05);H组大鼠卵巢AMH mRNA表达量较其它3组显著升高(P<0.05),M、L、C组间差异无统计学意义(P>0.05);H组大鼠卵巢中Kitlg和Fitla蛋白表达远远低于其它3组(P<0.05);H1foo蛋白表达L、M、H组显著低于C组(P<0.05),M、H组显著低于L组(P<0.05);AMH蛋白表达H、M组显著高于C、L组(P<0.05),H组则显著高于M组(P<0.05)。结论:青春期前雌性大鼠处于双酚A的环境中能够导致Kitlg基因启动子区甲基化程度升高,从而使得卵泡不能正常发育,造成大鼠生殖系统的损伤。
Objective: To investigate the effect of bisphenol A on the expression of follicular development-related genes in pre-adolescent female rats. Methods: 21-day-old Wistar rats were divided into H, M, L and C groups. The H, M and L groups were injected intraperitoneally with 160, 40 and 10 mg · kg -1 bisphenol A. Intraperitoneal injection of C, The same capacity of refined olive oil. One week after ovariectomized, ovarian tissues were collected for quantitative PCR detection and Western blot analysis. The expression of mRNA and protein of Kitlg, Figla, H1foo and AMH in ovarian tissue of each group were compared. Results: The expression of Kitlg and Fitla mRNA in ovary of H group was lower than that in C, L and M groups (P <0.05), but there was no significant difference between M, L and C groups (P> 0.05) The expression of H1foo mRNA in the ovary of the other three groups was significantly lower than that in the other three groups (P <0.05), but there was no significant difference between the groups of L, M and H (P> 0.05) (P <0.05). There was no significant difference between M, L and C groups (P> 0.05). The expression of Kitlg and Fitla protein in ovary of H group was much lower than that of the other three groups (P <0.05) ; The expression of H1foo protein in L, M, H group was significantly lower than that in C group (P <0.05), while M, H group was significantly lower than L group (P <0.05); AMH protein expression in H, M group was significantly higher than C, L group (P <0.05), H group was significantly higher than M group (P <0.05). CONCLUSION: Preadolescent female rats in the environment of bisphenol A can cause the methylation of Kitlg gene promoter region to increase, thus preventing the follicles from developing normally and causing damage to the reproductive system in rats.