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目的:探讨天麻对缺氧诱导大鼠大脑皮质神经元细胞凋亡的保护作用。方法:制备体外培养大鼠大脑皮层神经细胞缺氧模型,应用包括倒置显微镜下观察正常和缺氧神经细胞的生长情况、乳酸脱氢酶(LDH)释放率测定、流式细胞技术、TUNEL法的方法观察天麻对缺氧神经元细胞凋亡的保护作用。结果:缺氧处理后细胞LDH释放率测定显示,天麻各剂量组与尼莫地平组均可降低LDH的释放率,天麻低剂量组与尼莫地平组之间相比有明显差异(P<0.05)。流式细胞仪凋亡细胞记数:在缺氧处理前用天麻和尼莫地平预处理均可减少细胞的凋亡,天麻低剂量组与尼莫地平组之间相比有明显差异(P<0.05)。TUNEL法观察:在缺氧处理前用天麻和尼莫地平预处理均可抑制TUNEL阳性细胞的表达,天麻低剂量组与尼莫地平组之间相比有明显差异(P<0.05)。结论:天麻可明显减轻缺氧引起的大鼠大脑皮质神经细胞凋亡的发生,减轻细胞损伤,从而达到对缺氧神经细胞的保护作用。
Objective: To investigate the protective effect of Gastrodia elata on hypoxia-induced apoptosis of rat cerebral cortical neurons. Methods: The model of rat cerebral cortical neurons hypoxia was established. The changes of normal and hypoxic neurons were observed under an inverted microscope. The release of lactate dehydrogenase (LDH), flow cytometry, TUNEL method To observe the protective effect of Gastrodia elata on apoptosis of hypoxic neurons. Results: The LDH release rate of hypoxia treated group showed that the release rate of LDH in each dose group and nimodipine group was significantly lower than that in nimodipine group (P <0.05) ). Flow cytometry apoptotic cells count: pretreatment with gastrodin and nimodipine before hypoxia can reduce cell apoptosis, gastrodin low dose group and nimodipine group was significantly different (P < 0.05). TUNEL method: Pretreatment with gastrodin and nimodipine before hypoxia could inhibit the expression of TUNEL positive cells. There was significant difference between low dose Tianma group and nimodipine group (P <0.05). Conclusion: Gastrodia can obviously alleviate the hypoxia-induced neuronal apoptosis of rat cerebral cortex and reduce the cell injury so as to achieve the protective effect on hypoxic neurons.