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以苦苣为试材,采用营养液栽培法,研究了不同供氮水平对苦苣生长、根系活力、叶片叶绿素相对含量(SPAD值)、光合气体交换参数、H2O2和丙二醛(MDA)含量以及活性氧清除能力的影响。结果表明:在5个不同供氮处理水平中,8mmol·L-1(N8)的施氮水平下苦苣植株生长最佳,这与植株具有较高水平的根系活力、SPAD值、净光合速率(Pn)和较高的活性氧清除能力、较低的膜脂过氧化程度有关。而低氮(2、5mmol·L-1)或高氮供应水平(11、14mmol·L-1)因不同程度降低苦苣植株根系活力、叶片光合色素含量和活性氧清除能力,导致H2O2过量积累、膜脂过氧化加剧,细胞膜的结构及功能受损伤,从而抑制苦苣植株的光合作用和生长。
The experiment was carried out by using nutrient solution culture to study the effects of different nitrogen supply levels on the growth, root vigor, chlorophyll content (SPAD), photosynthetic gas exchange parameters, H2O2 and malondialdehyde (MDA) As well as the impact of reactive oxygen scavenging capacity. The results showed that under 5 nitrogenous fertilizer levels, 8 mmol·L-1 N8 had the best growth on the plants of bitter chinensis, which had higher root activity, SPAD value, net photosynthetic rate (Pn) was associated with higher reactive oxygen species scavenging ability and lower membrane lipid peroxidation. Low concentrations of nitrogen (2, 5mmol·L-1) and high nitrogen (11,14mmol·L-1) decreased root activity, photosynthetic pigment content and reactive oxygen scavenging ability of the plants, leading to excessive H2O2 accumulation , Membrane lipid peroxidation aggravated, cell membrane structure and function of injury, thereby inhibiting the photosynthesis and growth of plants.