论文部分内容阅读
A simple dual analyte fluorescein-based probe (PF3-Glc) was synthesised containing β-glucosidase (βglc) and hydrogen peroxide (H2O2) trigger units.The presence ofβ-glc,resulted in fragmentation of the parent molecule releasing glucose and the slightly fluorescent mono-boronate fluorescein (PF3).Subsequently,in the presence of glucose oxidase (GOx),the released glucose was catalytically converted to D-glucono-δ-lactone,which produced H2O2 as a by-product.The GOx-produced H2O2,resulted in classic H2O2-mediated boronate oxidation and the release of the highly emissive fluorophore,fluorescein.This unique cascade reaction lead to an 80-fold increase in fluorescence intensity.