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目的:探讨锌饱和乳铁蛋白(Zn2+-BLF)、铁饱和乳铁蛋白(Fe2+-BLF)体外抑制乙型肝炎表面抗原分泌作用,为锌、铁乳铁蛋白应用于临床治疗乙型肝炎病毒(HBV)感染提供理论和试验依据。方法:以天然HBV感染HepG2细胞为模型,通过应用ELISA法测定细胞上清中的HBsAg水平来检测Zn2+-BLF、Fe2+-BLF的抗HBsAg分泌效果,并用MTT法对Zn2+-BLF、Fe2+-BLF对HepG2细胞的毒性进行研究。结果:Zn2+-BLF、Fe2+-BLF对细胞的最大无毒剂量(TD0)分别为1.5g/L、3.0g/L;先用HBV对HepG2细胞进行感染,再分别加入Zn2+-BLF、Fe2+-BLF,各浓度组Zn2+-BLF均对HBsAg分泌有一定抑制作用,Zn2+-BLF浓度1.0g/L时对HBsAg的抑制率达60.49%;浓度为1.0g/L、0.5g/L的Fe2+-BLF能显著抑制HBsAg的分泌,但0.1g/L的Fe2+-BLF不能显著抑制HBsAg的分泌。结论:当HepG2细胞感染HBV后,Zn2+-BLF、Fe2+-BLF可以显著抑制HBsAg的分泌,Zn2+-BLF对HBsAg的抑制作用强于Fe2+-BLF,但其作用机理有待深入研究。
Objective: To investigate the inhibitory effect of zinc-saturated lactoferrin (Zn2 + -BLF) and iron-saturated lactoferricin (Fe2 + -BLF) on the secretion of hepatitis B surface antigen in vitro and to explore the potential clinical application of zinc and lactoferricin in the treatment of hepatitis B virus HBV) infection provide theoretical and experimental basis. Methods: HepG2 cells infected with natural HBV were used as a model to detect the anti-HBsAg secretion of Zn2 + -BLF and Fe2 + -BLF by ELISA. The levels of HBsAg in the supernatant of the cells were detected by MTT assay. The effects of Zn2 + -BLF, Fe2 + -BLF The toxicity of HepG2 cells was studied. Results: The maximum non-toxic dose (TD0) of Zn2 + -BLF and Fe2 + -BLF cells were 1.5g / L and 3.0g / L, respectively. HepG2 cells were infected with HBV before adding Zn2 + -BLF and Fe2 + -BLF , And the concentration of Zn2 + -BLF all inhibited the secretion of HBsAg. When the concentration of Zn2 + -BLF was 1.0g / L, the inhibition rate of HBsAg was 60.49%. The concentration of Fe2 + -BLF was 1.0g / L and 0.5g / L Significantly inhibited the secretion of HBsAg, but 0.1g / L of Fe2 + -BLF can not significantly inhibit the secretion of HBsAg. Conclusions: When HepG2 cells are infected with HBV, Zn2 + -BLF and Fe2 + -BLF can significantly inhibit the secretion of HBsAg. The inhibitory effect of Zn2 + -BLF on HBsAg is stronger than that of Fe2 + -BLF, but its mechanism needs further study.