应用凝胶过滤柱层析及逆相高效液相层析法,从冬鲽肝脏中分离提纯得到电泳单一纯的抗冻多肽B.用这种材料测定抗冻多肽浓度与额外冰点下降的关系,以双倒数法作图可得一直线,由此外推得K_(AP)值,K_(KP)的大小可作为抗冻多肽活性的反向指标.[H~+]对抗冻多肽B的影响,在动力学行为上表现为类非竞争性抑制类型.用甘氨酸甲酯对抗冻多肽B的羧基进行了化学修饰的动力学研究,表明羧基为其活性功能团,其三个二次羧基中仅有一个是必需基团. Gel filtration column chromatography and reverse phase high performance liquid chromatography were used to separate and purify the pure pure anti-freeze polypeptide B from the liver of the winter tilapia B. The relationship between the concentration of the anti-frozen polypeptide and the extra freezing point was measured with this material, The reciprocal index of the activity of antifreeze polypeptide can be obtained by extrapolating the K_ (AP) value with the double reciprocal method. [H ~ +] The kinetic behavior of non-competitive inhibition of the type of class.With glycine methyl ester of anti-frozen peptide B carboxyl chemical modification kinetic study showed that the carboxyl group as its active functional group of the three secondary carboxyl only One is an essential group.