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以茶叶修剪物制备的生物炭为试验材料,采集多年种植茶树的酸化土壤进行室内培养试验,探究以0.5%、1.5%、2.5%和3.5%的不同生物炭比例添加至茶园土壤中,对茶园土壤CO2和N2O气体排放、p H值和微生物群落的影响.结果表明:与空白对照处理相比,生物炭添加在短期内对CO2和N2O气体排放具有一定的促进作用,增强C、N的矿化率,但促进作用随着生物炭施用量的增加而减弱.不同生物炭处理对土壤p H值、脱氢酶及微生物生物量碳具有增加作用.检测土壤中不同标记的磷脂脂肪酸PLFA发现,添加1.5%的生物炭处理组中土壤磷脂脂肪酸含量最高,为(203.93±3.14)μg·g-1,与对照差异显著(P<0.05).其中16:0、14:0(细菌)、18:1ω9c(真菌)、10Me18:0(放线菌)标记含量较高,不同处理的单个磷脂脂肪酸含量差异显著(P<0.05).表明添加生物炭能改善茶园酸性土壤,提升土壤微生物生物量及微生物数量.
Taking biochar prepared from tea pruning materials as test materials, acidification soils cultivated for many years in tea plants were collected for in-house culture experiments to explore the effects of adding different bio-carbon ratios of 0.5%, 1.5%, 2.5% and 3.5% Soil CO2 and N2O emission, p H value and microbial community.The results showed that compared with the blank control treatment, the biochar addition could promote the emission of CO2 and N2O gas in a short period of time, and enhance the carbon and nitrogen ore mines However, the promotion effect was weakened with the increase of biochar application rate.The biochar treatment increased soil p H, dehydrogenase and microbial biomass carbon.Using PLFA of different labeled phospholipid fatty acids in soil, The content of soil phospholipid fatty acid was the highest (203.93 ± 3.14) μg · g-1 in the treatment with 1.5% biochar, which was significantly different from the control (P <0.05), including 16: 0,14: 0 : 1ω9c (fungi) and 10Me18: 0 (actinomycetes) were higher than those of the other treatments (P <0.05), indicating that the addition of biochar could improve the acidic soil of tea plantation, enhance the microbial biomass and Number of microorganisms.