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目的:探讨人参皂苷CK对大鼠心肌缺血再灌注损伤的保护作用及潜在机制。方法:SD大鼠随机均分为5组,分别为假手术组及缺血再灌注模型组给予生理盐水、人参皂苷CK低、中、高剂量组(10,20,40 mg·kg-1),每组12只,ip人参皂苷CK,14 d后,行在体结扎冠状动脉前降支手术30 min后,再进行120 min灌注,复制心肌缺血再灌注大鼠模型,检测人参皂苷CK对心肌梗死面积,血清乳酸脱氢酶(LDH),肌酸磷酸激酶(CPK),诱导型一氧化氮合酶(i NOS),白细胞介素-6(IL-6),肿瘤坏死因子-α(TNF-α)水平,心肌组织丙二醛(MDA)含量,超氧化物歧化酶(SOD)活性及高迁移率族蛋白1(HMGB1)蛋白表达的影响。结果:与正常组比较,缺血再灌注模型组心肌组织MDA含量明显升高,SOD活性明显降低,血清中i NOS,IL-6和TNF-α水平明显升高,心肌组织HMGB1蛋白的表达明显升高(P<0.05);与缺血再灌注模型组比较,人参皂苷CK能够明显减少心肌梗死面积,降低LDH,CPK活性减少心肌组织MDA含量,提高SOD活性,明显降低血清中i NOS,IL-6和TNF-α水平(P<0.05),此外,人参皂苷CK能够抑制心肌组织HMGB1蛋白的表达(P<0.05)。结论:人参皂苷CK对大鼠心肌缺血再灌注损伤具有一定的保护作用,该作用与其提高机体抗氧化能力、减少炎症反应及抑制HMGB1表达有关。
Objective: To investigate the protective effect and potential mechanism of ginsenoside CK on myocardial ischemia-reperfusion injury in rats. Methods: Sprague-Dawley rats were randomly divided into 5 groups: sham-operation group and ischemia-reperfusion model group were given saline, low, medium and high doses of ginsenoside CK group (10,20,40 mg · kg-1) , 12 rats in each group, ip ginsenoside CK, 14 days after the ligation of the coronary artery anterior descending branch surgery 30 min, and then 120 min perfusion, myocardial ischemia-reperfusion rat model was copied, the detection of ginsenoside CK Myocardial infarct size, serum LDH, CPK, iNOS, IL-6 and TNF-α (TNF-α), malondialdehyde (MDA), superoxide dismutase (SOD) activity and high-mobility group box 1 protein (HMGB1) Results: Compared with the normal group, the content of MDA in myocardium increased significantly and the activity of SOD decreased significantly in the ischemia / reperfusion model group. The levels of iNOS, IL-6 and TNF-α in the serum were significantly increased, and the expression of HMGB1 protein in the myocardium was obvious (P <0.05) .Compared with ischemia-reperfusion model group, ginsenoside CK could significantly reduce myocardial infarct size, decrease LDH and CPK activity, decrease myocardial MDA content, increase SOD activity and significantly decrease serum iNOS, IL -6 and TNF-α (P <0.05). In addition, ginsenoside CK could inhibit the expression of HMGB1 in myocardium (P <0.05). CONCLUSION: Ginsenoside CK has a protective effect on myocardial ischemia-reperfusion injury in rats, which may be related to enhancing the antioxidant capacity, reducing the inflammatory reaction and inhibiting the expression of HMGB1.