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Objective: To monitor the physiological characteristics and genes expression of obesity rat model after rambutan peel extract(RPE) treatment.Methods: Twenty-four 12-week-old male rats were divided into 4 groups: normal,obesity, obesity treated with ellagic acid(O-EA) and obesity treated with RPE30(ORPE30). Physiological characteristics were monitored by measuring body weight, calorie intake, size of adipocyte and level of triglyceride. Peroxisome proliferator activated receptor gamma(PPARg), CCAAT/enhancer-binding proteins a and fatty acid binding protein 4(FABP4) expression were observed using immunohistochemistry, Western blotting and quantitative RT-PCR methods.Results: Body weight gain of O-EA and O-RPE30 rats were lower than obesity group and size of adipocyte cells were smaller than obesity group(P < 0.05), but when we compared to normal group, those groups had higher body weight gain and larger adipocyte cells. The level of triglycerides, protein expression of PPARg and m RNA level of FABP4 genes were significantly downregulated on O-EA and O-RPE30 compared to obesity group(P < 0.05). Our results indicated that RPE had potential substance as inhibitor of body weight gain, declining of size of adipocyte, level of triglycerides, PPARg expression and m RNA level of FABP4 gene on obesity rat model.Conclusions: RPE have anti-obesity activity by inhibiting body weight gain, declining size of adipocyte, decreasing triglyceride, PPARg expression and m RNA level of FABP4 gene on obesity rat model.
Objective: To monitor the physiological characteristics and genes expression of obesity rat model after rambutan peel extract (RPE) treatment. Methods: Twenty-four 12-week-old male rats were divided into 4 groups: normal, obesity, obesity treated with ellagic acid Physiological characteristics of monitored by measuring body weight, calorie intake, size of adipocyte and level of triglyceride. Peroxisome proliferator activated receptor gamma (PPARg), CCAAT / enhancer-binding proteins a (O-EA) and obesity treated with RPE30 Results: Body weight gain of O-EA and O-RPE30 rats were lower than obesity group and size of adipocyte cells were smaller than obesity group (P <0.05), but when we compared to normal group, those groups had higher body weight gain and larger adipocyte cells. The level of triglycerides, protein expression of PPARg and m RNA le The vel of FABP4 genes were significantly downregulated on O-EA and O-RPE30 compared to obesity group (P <0.05). Our results indicated that RPE had potential substance as inhibitor of body weight gain, declining of adipocyte, level of triglycerides, PPARg expression and m RNA level of FABP4 gene on obesity rat model. Conclusions: RPE have anti-obesity activity by inhibiting body weight gain, declining size of adipocyte, decreasing triglyceride, PPARg expression and m RNA level of FABP4 gene on obesity rat model.