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单克隆抗体是近年发展起来的一种新技术。本实验应用酶标记抗体法,鉴定抗hCG单克隆抗体的特异性,并与抗hCG多克隆抗体比较,以积累使用单克隆抗体的经验。 取人早期妊娠(40～60天)胎盘绒毛、大鼠和小鼠垂体各4例,固定于4％多聚甲醛,4℃下孵育24小时。经磷酸缓冲液浸洗3天后,脱水、透明、石蜡包埋。另人垂体4例是我校病理教研室提供的、经10％福尔马林固定后石蜡包埋的组织块。全部标本均作厚5μ的连续切片,同时进行免疫组织化学染色。用作第一抗体的抗hCG多克隆抗体是我校第一医院制备
Monoclonal antibody is a new technology developed in recent years. In this experiment, the enzyme-labeled antibody method was used to identify the specificity of anti-hCG monoclonal antibody and compare it with anti-hCG polyclonal antibody in order to accumulate the experience of using monoclonal antibody. Take early pregnancy (40 to 60 days) placental villi, rat and mouse pituitary in 4 cases, fixed in 4% paraformaldehyde, incubated at 4 ° C for 24 hours. After 3 days immersion in phosphate buffer, dehydrated, transparent and embedded in paraffin. Another 4 cases of human pituitary gland is provided by the Department of Pathology, after 10% formalin fixed paraffin-embedded tissue blocks. All specimens were made thick 5μ serial sections, at the same time immunohistochemical staining. The anti-hCG polyclonal antibody used as the primary antibody was prepared by our First Hospital