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为建立检测人巨细胞病毒（ＨＣＭＶ）感染的ＰＣＲ方法并探讨ＨＣＭＶ与宫颈癌的关系，从石蜡包埋和活检宫颈癌组织中提取ＤＮＡ，用聚合酶链反应体外扩增ＨＣＭＶＥｃｏＲＩＤ片段上一段长１５２ｂｐ的核酸序列，再用地高辛末端转移酶标记法标记和扩增片段互补的ＨＣＭＶ寡核苷酸探针，用该探针证实扩增片段的特异性。检测宫颈癌标本４３例，阳性率为２５．６％（１１／４３），正常宫颈组织８例，阳性率为０。宫颈癌标本阳性率高于正常宫颈标本，提示ＨＣＭＶ与宫颈癌的发生有关。
To establish a PCR method for detection of human cytomegalovirus (HCMV) infection and to explore the relationship between HCMV and cervical cancer, DNA was extracted from paraffin-embedded and biopsy cervical cancer tissues, and a fragment of HCMVEcoRID was amplified by polymerase chain reaction The HCMV oligonucleotide probes complementary to the fragments were labeled and amplified by digoxigenin-terminal transferase, and the probe was used to confirm the specificity of the amplified fragment. 43 cases of cervical cancer specimens were detected, the positive rate was 25.6% (11/43), normal cervical tissue in 8 cases, the positive rate of 0. The positive rate of cervical cancer specimens than normal cervical specimens, suggesting that the occurrence of cervical cancer and HCMV.