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现介绍一种快速测定种子生活力的方法,即红墨水染色法: 一、操作方法 1、浸种:先将待测的种子用30~35℃温水浸泡1~2小时,待充分吸胀后备用。 2、切种:取吸胀的种子100粒,用刀片沿胚部中线切成两半,取其中一半用于测定生活力。 3、染色:将准备好的种子置于培养皿(或小盘中),再加入红墨水染色剂,以浸没种子为度。染色15~20分钟后倒出溶液,用水反复冲洗种子,直到所染的颜色不再被洗出为止。
Now introduce a rapid determination of seed viability method, that is, red ink staining: First, the operation method 1, soaking seeds: the first to be tested with 30 ~ 35 ℃ warm water soak for 1 to 2 hours until fully swollen back reserve . 2, cut: take swollen seeds 100, with a blade along the embryo midline cut in half, whichever is used to determine the viability. 3, Dyeing: Prepared seeds placed in Petri dishes (or small cap), then add red ink stain to the degree of immersion seeds. Dilute the solution 15 to 20 minutes later and rinse the seeds repeatedly with water until the dyed color is no longer washed out.