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目的:探讨有丝分裂原激活的蛋白激酶-细胞外调节激酶1/2(MAPKEERK1/2)信号通路在系统性红斑狼疮合并肾炎(LN)患者外周血单个核细胞(PBMC)中自发高水平表达白细胞介素6(IL-6)和抗双链DNA抗体(抗DS -DNA抗体)中的作用。方法:分离培养患者PBMC,利用蛋白免疫印迹法、逆转录PCR法和酶联免疫吸附法分别检测MAPKEHK1/2磷酸化活化程度、IL-6表达和抗DS-DNA抗体水平,并与正常对照组比较。结果:26例LN患者与21 例健康对照者比较,体外培养LN患者PBMC MAPKERK1/2信号通路呈高度活化状态,并自发表达高水平IL-6和抗DS -DNA抗体,组间有显著差异(P<0．05)。应用特异性阻断剂PD98059阻断LN患者PBMC MAPKERK1/2信号通路活化可显著抑制IL-6和抗DS-DNA抗体的自发高表达。结论:LN患者PBMC中MAPKERK1/2信号通路异常活化,并介导 PBMC自发高水平表达IL-6和抗DS-DNA抗体。
AIM: To investigate the expression of MAPKERK1 / 2 signaling pathway in peripheral blood mononuclear cells (PBMCs) from patients with systemic lupus nephritis (LN) (IL-6) and anti-double-stranded DNA antibodies (anti-DS-DNA antibodies). Methods: The PBMC of patients were isolated and cultured. The phosphorylation of MAPKEHK1 / 2, the expression of IL-6 and the level of anti-DS-DNA antibody were detected by Western blotting, reverse transcription PCR and enzyme-linked immunosorbent assay respectively. Compare Results: Compared with 21 healthy controls, the MAPKERK1 / 2 signaling pathway of PBMC was highly activated in LN patients and LN-6 and anti-DS-DNA antibodies were spontaneously expressed in 26 patients with LN. There was significant difference between the two groups P <0.05). Specific blockade of PD98059 blocked the activation of PBMC MAPKERK1 / 2 signaling pathway and significantly inhibited the spontaneous high expression of IL-6 and anti-DS-DNA antibodies. CONCLUSION: MAPKERK1 / 2 signaling pathway is abnormally activated in PBMC of LN patients and mediates spontaneous and high level expression of IL-6 and anti-DS-DNA antibodies in PBMCs.