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目的:验证肝脏是否存在局部肾素-血管紧张素-醛固酮系统,明确其与肝纤维化的关系。 方法:取(?)成年SD大鼠40只,随机分为4组。Ⅰ组为对照组,给予橄榄油皮下注射;Ⅱ～Ⅳ组为四氯化碳肝纤维化模型组,分别于2、4、6wk被处死。采用放射免疫法测定血液和肝匀浆中肾素、血管紧张素、醛固酮水平;采用紫外分光光度法测定血管紧张素转换酶活性;采用图像分析法判定肝纤维化程度。 结果:正常大鼠肝脏中可测到肾素活性、血管紧张素Ⅰ&Ⅱ、醛固酮和血管紧张素转换酶活性(分别为1.5±1.2μg/L/h,5.18±1.3μg/L,561.6±210.7ng/L,0.4±0.1μg/L,66.2±7.2μmol/L/min),并随肝纤维化进展逐渐升高(造模后6wk为4.3±1.8μg/L/h,13.1±2.4μg/L,762.5±322.5ng/L,0.6±0.2μg/L,96.2±17.8μmol/L/min)。 结论:肝脏存在局部肾素-血管紧张素-醛固酮系统,并随肝纤维化进展逐渐激活,说明此系统参与了肝纤维化形成。
PURPOSE: To verify the existence of a local renin-angiotensin-aldosterone system in the liver and determine its relationship with liver fibrosis. Methods: Forty adult SD rats were randomly divided into four groups. Group Ⅰ was given as control group and given subcutaneous injection of olive oil. Group Ⅱ ~ Ⅳ was model group with CCl 4 hepatic fibrosis, which were sacrificed at 2, 4 and 6 weeks respectively. The levels of renin, angiotensin and aldosterone in blood and liver homogenates were determined by radioimmunoassay. The activity of angiotensin converting enzyme was determined by ultraviolet spectrophotometry. The degree of hepatic fibrosis was evaluated by image analysis. RESULTS: Renin activity, angiotensin I, aldosterone and angiotensin converting enzyme activity (1.5 ± 1.2μg / L / h, 5.18 ± 1.3μg / L, 561.6 ± 210.7ng, /L0.4 ± 0.1μg / L, 66.2 ± 7.2μmol / L / min), and gradually increased with the progress of liver fibrosis (6wk after modeling 4.3 ± 1.8μg / L / h, 13.1 ± 2.4μg / L , 762.5 ± 322.5 ng / L, 0.6 ± 0.2 μg / L, 96.2 ± 17.8 μmol / L / min). CONCLUSIONS: There is a local renin-angiotensin-aldosterone system in the liver and is gradually activated as liver fibrosis progresses, indicating that this system is involved in the formation of hepatic fibrosis.