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HIV抗药性的产生严重阻碍了HIV疾病的治疗进程,因此开发新的抗HIV药物以及对病毒进行抗药性分析对于提高HIV疾病的治疗效果非常重要。将利用HIV假病毒构建的抗HIV药物评价系统及病毒抗药性分析系统应用于药物筛选及耐药性分析具有常规方法无法替代的优点。介绍了目前常用的几种类型HIV假病毒的特点和构建方法,同时介绍了假病毒感染细胞的系统及其在细胞水平筛选抗HIV药物和对HIV临床分离株进行耐药性分析这两方面的应用,并通过与常规方法进行比较来分析利用假病毒技术进行研究的优势及局限性,还通过总结大量学者的研究成果证明了利用假病毒技术进行药物筛选和抗药性分析具有准确、安全和高效的特点。
The emergence of HIV resistance has severely hampered the progress of HIV treatment, so the development of new anti-HIV drugs and drug resistance analysis is very important to improve the treatment effect of HIV disease. The HIV anti-HIV drug evaluation system and the virus resistance analysis system constructed by HIV pseudovirus are applied to drug screening and drug resistance analysis and can not be replaced by conventional methods. The characteristics and construction methods of several commonly used HIV pseudoviruses are introduced. Meanwhile, the system of pseudovirion-infected cells and their application in cell-level screening of anti-HIV drugs and drug resistance analysis of HIV clinical isolates are introduced Applications and by comparing with the conventional methods to analyze the advantages and limitations of the research using pseudoviruses, as well as the results of a large number of scholars have concluded that the use of pseudovirions for drug screening and drug resistance analysis is accurate, safe and efficient specialty.