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黑点银纹夜蛾(Plusia nigrisigna Walker)是一种分布广、食性杂的害虫。1979年8月从泰安市郊豆地采集自然患病死亡的虫尸中,分离获得一株核型多角体病毒。四年来,进行了病毒学及室内外感染试验。 在透射电镜下观察,黑点银纹夜蛾核多角体大小形态不一致,呈4、5、6边形和近圆形等多种形状,大小约0.7-1.8微米。根据对死虫称重后逐条进行多角体分离,运用加权平均测算每毫克体重中含有多角体数量约0.6×10~7个,每头6龄幼虫体重一般200毫克左右,多角体可达1.2×10~9个。
Plusia nigrisigna Walker is a widely distributed pest of food habits. August 1979 from the outskirts of Tai’an Beans collected natural dead worm body, isolated from a nuclear polyhedrosis virus. In the past four years, virological and laboratory tests have been conducted. Observed by transmission electron microscopy, the size and shape of Spodoptera frugiperda nuclear polyhedrosis are inconsistent, showing a variety of shapes such as 4, 5, 6 and nearly circular, the size of about 0.7-1.8 microns. According to the weight of dead insects one by one polyhedron separation, the use of weighted average weight per milligram body contains polyhedron number about 0.6 × 10 ~ 7, each 6th instar larvae body weight is generally about 200 mg, polyhedron up to 1.2 × 10 ~ 9.