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采用单管一步完成端粒重复序列扩增 (TRAP)法检测肝癌细胞 (HepG2 )经某豆科植物种子粗提物 (简称JA1)不同浓度作用前后和相同浓度、不同时间作用前后端粒酶活性的变化 ,并采用流式细胞仪检测细胞凋亡和细胞周期的改变。结果显示 ,JA1可显著抑制HepG2的端粒酶活性 ,而且这种抑制效果有浓度依赖性和时间依赖性。流式细胞仪分析表明 ,经JA1作用后的HepG2标本中有明显的DNA低含量颗粒 (“亚G1期”峰 ) ,表明JA1诱导了HepG2的凋亡 ,且凋亡率与JA1的浓度和作用时间呈正相关。
The Telomere Repetitive Sequence Amplification (TRAP) method was used to detect the telomerase activity of hepatocellular carcinoma cells (HepG2) before and after different concentrations of a legume plant seed extract (abbreviated as JA1) and before and after the same concentration and time. The changes and flow cytometry were used to detect apoptosis and cell cycle changes. The results show that JA1 can significantly inhibit the telomerase activity of HepG2, and this inhibitory effect is concentration- and time-dependent. Flow cytometry analysis showed that there was a low content of DNA (“sub-G1 phase” peak) in HepG2 samples after JA1 treatment, indicating that JA1 induced apoptosis of HepG2, and the apoptosis rate and the concentration and role of JA1 The time is positively correlated.