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目的：研究维生素Ｃ对中脑神经细胞生长功能的影响。方法：采用大鼠胚胎中脑神经细胞进行体外培养，观察不同浓度（０．００１～１００ｍｏｌ／Ｌ）维生素Ｃ（ＶＣ）对中脑细胞生长发育的影响。结果：培养５天的细胞存活数在０．００１－０．１ｍｏｌ／Ｌ，ＶＣ组与对照组之间无明显差别，１０ｍｏｌ／ＬＶＣ组中脑神经细胞集落数目、长度、胞体面积、细胞蛋白质相对含量明显高于对照组（Ｐ＜０．０５），而５０～１００ｍｏｌ／ＬＶＣ组存活的细胞数明显降低。结论：１０ｍｏｌ／ＬＶＣ对体外培养中脑神经细胞的生长发育有一定的促进生长分化作用。
Objective: To study the effect of vitamin C on the growth of neural cells in midbrain. METHODS: Rat embryonic midbrain neurons were cultured in vitro and the effects of different concentrations (0.001 ~ 100mol / L) of vitamin C on the growth and development of mesencephalic cells were observed. Results: The number of viable cells cultured for 5 days was 0.001-0.1 mol / L, there was no significant difference between VC group and control group. The number, length, cell body area and cell protein of midbrain neurons in 10 mol / LVC group were Content was significantly higher than the control group (P <0.05), while the number of cells in the 50 ~ 100mol / LVC group decreased significantly. Conclusion: 10mol / LVC can promote the growth and differentiation of midbrain nerve cells in vitro.