我国现用布氏活菌苗为皮肤划痕。我们曾试用滴鼻免疫法,共接种1000余人,分滴鼻50亿菌、滴鼻1亿菌和皮肤划痕50亿菌三个组,种后1、3、9和12个月用试管凝集和皮肤变态反应作阳转率观察,阳转率分别为50亿滴鼻组-74.0～91.5％;1亿滴鼻组—45。2～82.9％;50亿划痕组——37.2～65.5％。种后5～60天部份人中分离7株菌苗菌种,经动物试 Mycotoxin live vaccine is currently used for skin scratches. We have tried nasal immunization, a total of more than 1,000 were inoculated, sub-nasal 5 billion bacteria, 100 million drops of nose and skin scars 5 billion bacteria in three groups, 1, 3, 9 and 12 months after the test tube agglutination And skin allergy as positive conversion rate, the positive conversion rate was 5 billion nasal drops -74.0-91.5%; 100 million nasal drops -45.2-82.9%; 5 billion scratches -37.2-65.5% . After 5 to 60 days after planting, seven strains of bacteria and bacteria were isolated from some people and tested by animals