论文部分内容阅读
目的:探讨慢性肾脏病5D期(Chronic Kidney Disease stage 5D,CKD5D)患者体内氧化应激水平变化及相关影响因素,比较不同的肾替代治疗方式对终末期肾病患者体内氧化应激水平的影响。方法:收集CKD5D期患者血清,采用双抗体夹心法测定血清超氧化物歧化酶(SOD)、丙二醛(MDA)和谷胱甘肽-过氧化物酶(GSH-Px)水平,同时收集患者临床资料,用统计学方法进行相关影响因素分析。结果:透析患者体内氧化应激水平明显高于正常对照组(P<0.05)。维持性血液透析患者氧化应激水平高于腹膜透析患者(P<0.05)。多因素回归分析结果提示:CKD5D期患者体内氧化应激水平与透析方式、透析时间、性别、年龄、是否贫血、他汀类药物、血管紧张素转化酶抑制剂/血管紧张素II受体阻滞剂(ACEI/ARB)类药物的使用及CRP密切相关(P<0.05)。而铁剂、重组人促红细胞生成素(EPO)的使用和白细胞计数与氧化应激水平无独立相关性(P>0.05)。结论:CKD5D期患者体内氧化应激水平与透析方式、透析龄和性别相关,维持性血液透析患者体内氧化应激水平高于维持性腹膜透析患者。
Objective: To investigate the changes of oxidative stress in patients with Chronic Kidney Disease Stage 5D (CKD5D) and its related factors, and to compare the effects of different renal replacement therapy on oxidative stress in patients with end-stage renal disease. Methods: The serum of patients with CKD stage 5D was collected. The levels of serum superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) were measured by double antibody sandwich method. Clinical data, using statistical methods for the analysis of influencing factors. Results: The level of oxidative stress in dialysis patients was significantly higher than that in the normal control group (P <0.05). Oxidative stress in maintenance hemodialysis patients was higher than that in peritoneal dialysis patients (P <0.05). Multivariate regression analysis showed that the level of oxidative stress in CKD5D patients was positively correlated with the dialysis method, dialysis time, sex, age, anemia, statins, angiotensin converting enzyme inhibitor / angiotensin II receptor blocker (ACEI / ARB) and CRP (P <0.05). The use of iron, recombinant human erythropoietin (EPO) and leukocyte count had no correlation with oxidative stress (P> 0.05). Conclusion: The level of oxidative stress in CKD5D patients is related to the mode of dialysis, dialysis age and sex, and the level of oxidative stress in maintenance hemodialysis patients is higher than that in maintenance peritoneal dialysis patients.