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观察补肾醒脑解毒中药复方对 HSV- 1感染后神经元内 NGF、BDNF表达的调节作用。用 HSV- 1感染原代培养大鼠海马神经元造成急性感染与潜伏感染 ,然后运用 Northern杂交方法检测中药煎液对感染神经元 NGF和 m RNA表达水平的影响。结果显示 :(1)正常培养海马神经元内有微量 NGF和 BDNF表达 ,加入中药煎液后 NGF和 BDNF表达明显提高 ;(2 )感染 0 .1MOI HSV- 1后 6 - 12 h出现 BDNF与 NGF表达增加 ,2 4 h后下降 ,4 8h后显著低于正常水平 ,4 d后表达消失。加中药煎液后 NGF和 BDNF表达时间延长 ;(3)补肾醒脑解毒方能使潜伏感染神经元 NGF和 BDNF表达持续增加 ,并维持潜伏感染神经元存活达 10周以上。结果表明补肾醒脑解毒方对 HSV- 1感染神经元 NGF和 BDNF表达具有上增性调节作用
Observe the regulation effect of Bushen Xingnao Detoxification Chinese Herbal Compound Compound on the expression of NGF and BDNF in neurons after HSV-1 infection. Infection of primary cultured rat hippocampal neurons with HSV-1 caused acute infection and latent infection. Northern hybridization was used to detect the effect of Chinese herb decoction on the expression levels of NGF and m RNA in infected neurons. The results showed that: (1) There were traces of NGF and BDNF expression in normal cultured hippocampal neurons, and the expression of NGF and BDNF increased significantly after adding Chinese herbal decoction; (2) BDNF and NGF occurred 6 to 12 hours after infection with 0.1 MOI HSV-1. The increase of expression decreased after 24 hours and was significantly lower than normal after 48 hours. The expression disappeared after 4 days. NGF and BDNF expression time prolonged after adding Chinese traditional medicine decoction; (3) Bushen Xingnao Jiedu can increase the expression of NGF and BDNF in latently infected neurons and maintain the survival of latently infected neurons for more than 10 weeks. The results showed that Bushen Xingnao Jiedu Decoction has an up-regulation effect on the expression of NGF and BDNF in HSV-1 infected neurons.