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[目的]探讨新城疫病毒(NDV)7793对结肠癌肝转移瘤小鼠肝组织Th1、Th2、Th17类细胞因子表达的影响,为NDV7793作用人结肠癌肝转移的免疫效果研究提供实验依据。[方法 ]取40只健康BALB/c小鼠,随机分为4组(每组10只):PBS组,5-Fu组,NDV7793组,正常对照组。建模及给药结束,采用ELISA法测定肝脏组织上清IL-2、TNF-α、IL-4、IL-10、IL-17的浓度,比较4组间各细胞因子表达差异,分析TNF-α与IL-17表达水平的相关性。[结果]经NDV7793治疗后,结肠癌肝转移瘤小鼠肝组织Th1、Th2、Th17表达均不同程度恢复到正常对照组水平,即Th1类细胞因子IL-2、TNF-α和Th17类细胞因子IL-17表达显著回升,Th2类细胞因子IL-4、IL-10表达显著下降;与5-Fu组相比,NDV7793组的小鼠肝脏各类细胞因子更趋近于正常对照组小鼠的水平;TNF-α表达水平与IL-17表达水平相关性检测,0.749≤r<1,P<0.01,有统计学意义。[结论 ]NDV7793能促进结肠癌肝转移小鼠的肝组织Th1类细胞因子分泌,抑制Th2类细胞因子分泌,使其趋近于正常水平,且小鼠TNF-α与IL-17表达呈正相关。
[Objective] To investigate the effect of Newcastle disease virus (NDV) 7793 on the expression of Th1, Th2 and Th17 cytokines in the liver tissue of mice with liver metastases of colon cancer and provide the experimental evidence for the study on the immune effect of NDV7793 on the liver metastasis of human colon cancer. [Method] Forty healthy BALB / c mice were randomly divided into 4 groups (10 in each group): PBS group, 5-Fu group, NDV7793 group and normal control group. The levels of IL-2, TNF-α, IL-4, IL-10 and IL-17 in the supernatant of the liver tissue were measured by ELISA. The expression of TNF- α and IL-17 expression level of the correlation. [Results] After treatment with NDV7793, the expression of Th1, Th2 and Th17 in liver tissues of mice with liver metastasis of colorectal cancer all returned to the level of normal control group, that is, the levels of Th1 cytokines IL-2, TNF-α and Th17 cytokines Compared with 5-Fu group, all kinds of cytokines in liver of NDV7793 group were more close to those in normal control group mice The level of TNF-α was correlated with the level of IL-17, with a statistical significance (0.749≤r <1, P <0.01). [Conclusion] NDV7793 can promote the secretion of Th1 cytokines and inhibit the secretion of Th2 cytokines in liver tissues of mice with liver metastases of colorectal cancer, and close to the normal level. The expression of TNF-α and IL-17 in mice is positively correlated.