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目的：探讨溃疡性结肠炎（ＵＣ）患者外周血Ｔ细胞亚群及γδ－Ｔ细胞的表达在ＵＣ发病中的作用。方法：用流式细胞仪双染法检测１３例正常人、１０例活动性溃疡性结肠炎（ＡＵＣ）及１２例非活动性溃疡性结肠炎（ＩＡＵＣ）患者外周血及ＰＨＡ刺激后的Ｔ细胞亚群及γδ－Ｔ细胞的表达。结果：ＰＨＡ刺激后，ＣＤ４＋细胞在ＡＵＣ及ＩＡＵＣ组均较对照组显著增加（Ｐ均＜０．０５）；ＡＵＣ及ＩＡＵＣ组间差异无显著性，但ＡＵＣ组的ＣＤ＿４￣＋Ｌｅｕ８￣－（辅助细胞）较ＰＨＡ刺激前显著增加（Ｐ＜０．０５）；γδ－Ｔ细胞（即ＴＣＲδｌ￣＋ＣＤ＿３￣＋）在ＡＵＣ组较对照组及ＩＡＵＣ组均显著减少（Ｐ均＜０．０５），而－Ｔ细胞的部分抗原表达（即ＴＣＲδ１￣＋ＣＤ＿３￣＋）在ＡＵＣ组及ＩＡＵＣ组均较对照组显著减少（Ｐ分别＜０．０１，＜０．０５），ＡＵＣ及ＩＡＵＣ组间差异无显著性；外周血ＴＣＲδ１￣＋ＣＤ＿４￣＋占总ＴＣＲδ１￣＋细胞百分数在ＡＵＣ组较对照组及ＩＡＵＣ组均显著增加（Ｐ均＜０．０５），ＰＨＡ刺激后该细胞在ＡＵＣ及ＩＡＵＣ组较对照组显著增加（Ｐ均＜０．０５）·结论：ＵＣ患者γδ－Ｔ细胞的增殖性降低，可能反映了γδ－Ｔ细胞对外来抗原免疫监视作用?
Objective: To investigate the role of peripheral blood T cell subsets and γδ-T cells in the pathogenesis of UC in patients with ulcerative colitis (UC). Methods: Flow cytometry was used to detect peripheral blood of 13 normal subjects, 10 patients with active ulcerative colitis (AUC) and 12 patients with inactive ulcerative colitis (IAUC) and T cells stimulated by PHA Subpopulations and γδ-T cells. Results: After stimulated with PHA, the percentage of CD4 + cells in AUC and IAUC groups increased significantly (P <0.05), but there was no significant difference between AUC and IAUC groups. However, CD_4 + Leu8- (P <0.05); γδ-T cells (TCRδl ~ + CD_3 ~ +) in AUC group were significantly decreased compared with control group and IAUC group (all P <0.05) The expression of T antigen (TCRδ1 ~ + CD_3 ~ +) in AUC group and IAUC group was significantly lower than that in control group (P <0.01, <0.05, respectively) Peripheral blood TCRδ1 ~ + CD_4 ~ + total TCRδ1 ~ + cell percentage in AUC group than the control group and IAUC group were significantly increased (P all <0.05), PHA stimulation in the AUC and IAUC group compared with the control group increased significantly (P <0.05 respectively) · Conclusion: γδ-T cell proliferation in patients with UC decrease may reflect the foreign antigens, immune surveillance γδ-T cells?