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目的 :观察四毒清对内毒素性肝损伤的影响及作用机制。方法 :配制中药复方四毒清 ( SDQ)为 1 g/ m L生药 ,小鼠随机分 4组 :对照组、内毒素组、SDQ组、SDQ防治组。用 H2 O或 SDQ灌胃 ,3d后腹腔注射 LPS或生理盐水。腹腔内注射不同时间后 ,观察小鼠血清 ALT的活性、肝组织匀浆中 TNF-α的含量、MDA的含量及 SOD的活性 ,且观察各组小鼠肝脏组织病理变化。结果 :腹腔LPS注射后小鼠血清 AL T活性及肝组织中 TNF-α、MDA的含量明显高于对照组 ;SDQ防治组小鼠血清 ALT活性及肝组织中 TNF-α、MDA的含量明显低于内毒素组 ,肝组织中 SOD活性明显高于内毒素组。组织学发现 SDQ防治组小鼠肝脏的病理明显减轻。结论 :中药复方 SDQ对肝脏具有一定的保护作用 ,其机制与其抑制 TNF-α和氧自由基生成有关
Objective: To observe the effect and mechanism of Siduqing on endotoxic liver injury. Methods: Preparation of traditional Chinese medicine compound Si Du Qing (SDQ) 1 g / m L crude drug, mice were randomly divided into 4 groups: control group, endotoxin group, SDQ group, SDQ prevention group. The rats were intragastrically administrated with H2O or SDQ and intraperitoneally injected with LPS or saline after 3 days. After intraperitoneal injection for different times, the activity of serum ALT, the content of TNF-α in liver tissue homogenate, the content of MDA, and the activity of SOD were observed, and the histopathological changes of liver in mice were observed. RESULTS: Serum AL T activity and TNF-α and MDA levels in liver tissue were significantly higher in mice after intraperitoneal LPS injection than in the control group. Serum ALT activity in the SDQ-treated mice and TNF-α and MDA levels in the liver tissue were significantly lower. In endotoxin group, SOD activity in liver tissue was significantly higher than in endotoxin group. Histologically, the pathological changes of the liver of SDQ-treated mice were significantly reduced. Conclusion : SDQ has a protective effect on liver and its mechanism is related to the inhibition of TNF-α and oxygen free radical production.