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目的观察中链甘油三酯(MCT)对血清和下丘脑瘦素、神经肽Y(NPY)浓度,下丘脑长型瘦素受体(Ob-Rb)、mRNA表达的影响。方法20只Wistar雄性大鼠,用含30%MCT或长链甘油三酯(LCT)的饲料喂养8周,用ELISA法测定血清和下丘脑瘦素的水平,用放射免疫法检测外周血和下丘脑NPY的浓度,用real-time RT-PCR技术测定下丘脑NPY、瘦素受体(Ob-Rb)基因表达水平的差异。结果MCT组大鼠体重增长量和体脂含量均小于LCT组(P<0.01),MCT组瘦素脑/血比明显高于LCT组,MCT组大鼠下丘脑ob-Rb mRNA表达水平明显高于LCT组(P<0.01),下丘脑及血清NPY浓度明显低于LCT组(P<0.01)。结论MCT通过促进Ob-Rb基因表达,抑制NPY合成和分泌。
Objective To observe the effects of medium chain triglyceride (MCT) on the levels of serum and hypothalamic leptin, neuropeptide Y (NPY), and Ob-Rb mRNA and mRNA in hypothalamus. Methods Twenty male Wistar rats were fed with 30% MCT or long-chain triglyceride (LCT) for 8 weeks. Serum and hypothalamic leptin levels were measured by ELISA. Peripheral blood and lower The concentrations of NPY in hypothalamus were determined by Real-time RT-PCR. The expression of NPY and Ob-Rb in hypothalamus were determined by real-time RT-PCR. Results The body weight gain and body fat content in MCT group were lower than that in LCT group (P <0.01). The leptin brain / blood ratio in MCT group was significantly higher than that in LCT group. The mRNA expression level of ob-Rb in hypothalamus of MCT group was significantly higher In LCT group (P <0.01), the concentrations of NPY in hypothalamus and serum were significantly lower than those in LCT group (P <0.01). Conclusion MCT can inhibit the synthesis and secretion of NPY by promoting Ob-Rb gene expression.