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目的:评价丙泊酚静脉麻醉对行取卵术患者自然杀伤（natural killer, NK）细胞的数量及妊娠结局的影响。方法:选择择期接受体外受精-胚胎移植（in vitro fertilization and embryo transfer, IVF-ET）的患者110例，年龄20~40岁，ASA分级Ⅰ、Ⅱ级，采用随机数字表法将患者分为2组（每组55例）：丙泊酚无痛取卵组（P组）和布洛芬组（C组）。术前收集患者一般临床资料，患者入室后监护生命体征，建立静脉通路。P组麻醉诱导方法为静脉缓慢推注丙泊酚2.0~2.5 mg/kg，待患者Ramsay镇静评分达6分后开始手术，丙泊酚8~12 mg·kgn -1·hn -1静脉泵注维持麻醉；C组术前15 min于阴道深部置入布洛芬栓剂。记录两组患者年龄、BMI、不孕年限、手术时间、ASA分级、所用超促排卵用药的天数、促排卵用药总量、基础卵泡刺激素（follicle-stimulating hormone, FSH）、黄体生成素（luteinizing hormone, LH）、雌二醇（estradiol, E2）水平。观察并记录患者在麻醉诱导前（Tn 0）、手术开始即刻（Tn 1）、手术结束即刻（Tn 2）、术后10 min（Tn 3）4个时间点的MAP、心率、SpOn 2，Tn 3时的VAS疼痛评分及不良反应发生情况；收集Tn 0、Tn 2、术后第1天晨（Tn 4）、胚胎移植前（Tn 5）静脉血，利用流式细胞仪检测NK细胞数量；记录两组患者妊娠结局情况。n 结果:P组MAP、心率在Tn 1、Tn 2、Tn 3时低于C组（n P<0.05）。与Tn 0比较：P组MAP及心率在Tn 1时升高，Tn 3时降低（n P<0.05）；C组MAP及心率在Tn 1、Tn 2、Tn 3时均升高（n P0.05）。P组Tn 3时VAS疼痛评分低于C组，术中体动例数少于C组（n P0.05）。P组Tn 2时NK细胞数量少于C组（n P<0.05）；与Tn 0比较，P组Tn 2时NK细胞数量减少（n P0.05）；P组获卵数、获卵率高于C组（n P<0.05）。n 结论:丙泊酚静脉麻醉用于取卵术能够降低患者NK细胞的数量，虽对单次妊娠结局无显著促进作用，但可以取得更高的获卵率，对后续妊娠结局有促进作用。“,”Objective:To evaluate the effects of intravenous anesthesia with propofol on the number of natural killer (NK) cells and pregnancy outcome of patients undergoing oocyte retrieval.Methods:According to the random number table method, 110 patients, aged 20 to 40 years, American Society of Anesthesiologists (ASA) Ⅰ or Ⅱ, who were scheduled for in vitro fertilization and embryo transfer (IVF-ET) were enrolled and divided into two groups (n n=55): a propofol group (group P) and a ibuprofen group (group C). Their general clinical data were collected before operation; the vital signs were monitored after entry into the operating room and venous access was established. Group P was intravenously infused with 2.0-2.5 mg/kg of propofol and the operation started when the score of sedation reached 6, with anesthesia maintenance with propofol at 8-12 mg·kgn -1·hn -1. Group C was vaginally placed with ibuprofen suppository 15 min before operation. Then, their age, body mass index (BMI), the duration of infertility, operation time, ASA grades, the days of superovulation, total dose of ovulation induction, and the levels of basal follicle-stimulating hormone (FSH), luteinizing hormone (LH) and estradiol (E2) were recorded. The mean arterial pressure (MAP), heart rate and pulse oxygen saturation (SpOn 2) were recorded before anesthesia induction (Tn 0), immediately after operation (Tn 1), immediately after operation (Tn 2) and 10 min after operation (Tn 3), VAS scores at Tn 3 and adverse reactions were recorded. The venous blood samples were collected at Tn 0, Tn 2, day 1 after operation (Tn 4) and before embryo transfer (Tn 5) to measure the number of NK cells by flow cytometry. Their pregnancy outcomes were recorded.n Results:MAP and heart rate in group P were lower than those in group C at Tn 1, Tn 2 and Tn 3 (n P<0.05). Compared with those at Tn 0, MAP and heart rate in group P increased at Tn 1 and decreased at Tn 3 (n P<0.05), and MAP and heart rate in group C increased at Tn 1, Tn 2 and Tn 3 (n P0.05). The VAS score in group P was lower than that in group C at Tn 3, and the number of movement cases in group P was lower than that in group C (n P0.05). The number of NK cells in group P was less than that in group C at Tn 2 (n P<0.05). The number of NK cells decreased at Tn 2 compared with Tn 0 in group P (n P0.05). The number of oocytes and oocyte retrieval rate in group P were higher than those in group C (n P<0.05).n Conclusions:Intravenous anesthesia with propofol can reduce the number of NK cells during oocyte retrieval operation. Although it does not significantly improve the outcome of single pregnancy, it can achieve a higher oocyte retrieval rate and promote the outcome of subsequent pregnancy.