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本文用RAPD方法对中国东北落叶松属植物的亲缘关系进行了研究。从100个引物中筛选出41个引物,检测出120个多态位点。应用UPGMA法计算了种群内和种群间的遗传距离,并构建了系统树。实验结果表明,分布在长白山的落叶松与分布在大、小兴安岭的落叶松之间的差异,在遗传距离上还不能达到种间分化水平。分布在长白山的落叶松应视为兴安落叶松种下的变种,分布在东京城的落叶松可能是兴安落叶松与长白山落叶松的杂交种。
In this paper, RAPD method was used to study the genetic relationship of Larix principis-rupprechtii in China. Thirty-one primers were screened out from 100 primers and 120 polymorphic loci were detected. The UPGMA method was used to calculate the genetic distance within and between populations and to construct a phylogenetic tree. The experimental results show that the difference between the larch distributed in Changbai Mountain and the larch distributed in Xiaoxing’an Mountains can not reach the level of interspecific differentiation in the genetic distance. The larch distributed in Changbai Mountain should be regarded as a variant under Larix gmelinii. The larch distributed in Tokyo may be the hybrid between Larix gmelinii and Larix gmelinii.