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用MTT比色法和细胞计数法测定细胞增殖。观察了Ge-132(羧乙基锗倍半氧化物)在体外对兔结膜成纤维细胞增殖的影响。结果显示:Ge-132药物最终浓度在0.0016—5mg/ml范围有剂量依从性的抑制成纤维细胞增殖的作用,其半数有效浓度(IC50)为0.147mg/ml。它有可能用于控制成纤维细胞的过度增殖。
Cell proliferation was measured by MTT colorimetric and cell counting methods. The effect of Ge-132 (carboxyethyl germanium sesquioxide) on the proliferation of rabbit conjunctival fibroblasts was observed in vitro. The results showed that dose-dependent inhibition of fibroblast proliferation was observed at the final concentration of Ge-132 in the range of 0.0016-5mg / ml. The IC50 was 0.147mg / ml. It is possible to control the excessive proliferation of fibroblasts.