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以褐黄孢链霉菌株(Streptomyces gilvosporeus)SG-17为出发菌株,依次利用紫外线、微波和Co60-γ射线进行诱变处理。通过致死率与诱变剂量的相互关系,确定最佳诱变剂量。采用80 s的紫外线照射、微波处理50 s、剂量为1.5 kGy的γ射线对褐黄孢链霉菌株S-17依次进行诱变,获得1株褐黄孢链霉突变菌株γ-188,突变株产纳他霉素的能力显著提高,发酵效价达到4.58 g/L,较出发菌株SG-17提高了1.45倍。紫外线、微波和Co60-γ射线三者的协同诱变效果好,突变株稳定性和产纳他霉素情况良好。
The strain of Streptomyces gilvosporeus SG-17 was mutagenized by UV, microwave and Co60-γ rays in turn. The optimal mutagenic dose was determined by the correlation between lethality and mutagenic dose. The mutant of T. aureus strain S-17 was mutagenized with 80 s UV irradiation, microwave irradiation for 50 s and irradiation dose of 1.5 kGy. The ability to produce natamycin was significantly improved. The fermentation titer reached 4.58 g / L, which was 1.45 times higher than that of the original strain SG-17. The synergistic mutagenesis effects of UV, microwave and Co60-γ rays are good, and the stability of mutants and the production of natamycin are good.