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冰冻切片快速、方便、用途广泛,但技术难度远较石蜡切片大,尤其是质量难以控制:我们经过多年实践,摸索出了一套外检急诊保质简易方法,现介绍如下。 1.方法 冰冻:将厚0.2～0.4mm新鲜组织放入预先滴上合成胶水(金雀牌,启东北新光卫胶水厂生产)作包埋剂的冰冻头上,放入恒温式冰冻切片机内,冰冻致组织和包埋剂发白后即可切片。 切片:切片时手摇轮用力要均匀,防卷板与切片刀锋之间的距离调节适当,这是获得平整切片的关键。附贴切片动作要轻巧而快,贴后立即用95％酒精固定30秒钟。
Frozen section is fast, convenient and versatile, but the technical difficulty is far greater than the paraffin section, especially the quality is difficult to control: After years of practice, we explored a set of simple method of quality assurance of emergency inspection, are described below. Method Frozen: The fresh tissue 0.2 ~ 0.4mm thick into the pre-drop synthetic glue (Jinque brand, Kai light northeastern Xinguang glue factory production) as the embedding agent frozen head, into the thermostatically frozen section machine , Frozen tissue and embedding agent can be sliced after whitening. Slice: Stiffness when slicing force to be uniform, anti-curling plate and slice the appropriate distance between the blade, which is the key to obtain a smooth slice. Affixed slice action should be lightweight and fast, paste immediately with 95% alcohol for 30 seconds.