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背景:许多体外实验研究表明低剂量氦氖激光可刺激细胞生长和促进血管再生,从而改善缺血所致的损害,而体内试验需进一步研究。目的:研究低剂量氦氖激光血管内照射对大鼠脑缺血再灌注的保护作用,探讨其作用机制。设计:以实验动物为研究对象,随机对照研究。单位:一所大学医院的神经内科。材料:实验于2003-09/2004-02在四川大学华西医院外科动物实验中心完成。健康雄性SD大鼠,二级动物,五六月龄,体质量372～418g(388.48±10.57)g,由四川大学动物研究中心提供。干预:采用大鼠大脑中动脉缺血(MCAO)模型,将40只Sprague-Dawley(SD)大鼠随机分成治疗组和对照组,每组20只。治疗组术后予以低剂量氦氖激光(2mW)血管内照射,20min/次,隔日1次,3次为1个疗程。对照组同样予以静脉穿刺,但无激光输出。主要观察指标:脑梗死体积比。结果:低剂量氦氖激光组血管内照射组脑梗死体积比10.43±1.04,明显低于对照组16.78±1.12,差异有统计学意义(t=27.14,P<0.05)。结论:低剂量氦氖激光血管内照射对大鼠脑缺血有保护作用。
Background: Many in vitro experimental studies have shown that low-dose He-Ne laser can stimulate cell growth and promote angiogenesis, thereby improving the damage caused by ischemia, and in vivo testing needs further study. Objective: To study the protective effect of low-dose He-Ne laser intravascular irradiation on cerebral ischemia-reperfusion in rats and its mechanism of action. Design: The experimental animals for the study, a randomized controlled study. Unit: Neurology of a university hospital. MATERIALS: The experiment was performed at the Experimental Animal Center of West China Hospital, Sichuan University from September 2003 to February 2004. Healthy male SD rats, two animals, five or six months old, body weight 372 ~ 418g (388.48 ± 10.57) g, provided by the Sichuan University Animal Research Center. Intervention: Forty Sprague-Dawley (SD) rats were randomly divided into treatment group and control group, 20 rats in each group. The rat middle cerebral artery occlusion (MCAO) model was used. The treatment group was given low-dose He-Ne laser (2mW) intravascular irradiation, 20min / time, every other day, 3 times for a course of treatment. The control group was also venipuncture, but no laser output. MAIN OUTCOME MEASURES: Volume ratio of cerebral infarction. Results: Compared with the control group, the infarct volume of the low-dose He-Ne laser group was 10.43 ± 1.04, significantly lower than that of the control group (16.78 ± 1.12, t = 27.14, P <0.05). Conclusion: Intravascular irradiation with low dose He-Ne laser can protect cerebral ischemia in rats.