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目的 检测原发性前列腺癌及高级别前列腺上皮内瘤 (PIN) 6号染色体等位基因杂合子丢失 (LOH)及其意义。方法 经显微切割技术获取前列腺癌及PIN各 10例患者DNA ,采用聚合酶链反应 (PCR)及微卫星多态性技术 ,对 6号染色体上的 2 0个微卫星标志位点LOH进行检测。结果 10例原发性前列腺癌中有 8例在 6号染色体上至少有 1个位点检测到LOH ,6q2 1 6q2 3及 6q2 5 6q2 7为2个高频LOH区。 10例高级别PIN检测 6号染色体 2 0个位点 ,有 5例各有 1个位点检测到LOH。结论 前列腺癌中存在 6号染色体的高频LOH区 ,分别位于 6q2 1 6q2 3、6q2 5 6q2 7区 ,编码细胞周期素C及胰岛素样生长因子Ⅱ受体的基因为此 2区候选的抑癌基因 ,它们可能与前列腺癌的发生发展有关。
Objective To investigate the significance of loss of heterozygosity (LOH) on chromosome 6 of primary prostate cancer and high grade prostatic intraepithelial neoplasia (PIN). Methods DNA from 10 patients with prostate cancer and PIN was obtained by microdissection. The microsatellite markers LOH of 20 chromosomes on chromosome 6 were detected by polymerase chain reaction (PCR) and microsatellite DNA polymorphism . Results In 10 cases of primary prostate cancer, LOH was detected in at least 1 locus on chromosome 6, and 2 high frequency LOH regions were found in 6q2 1 6q2 3 and 6q2 5 6q2 7. Ten high-grade PINs detected 20 chromosomes on chromosome 6, 5 of which had 1 locus detected LOH. Conclusions The high frequency LOH region of chromosome 6 in prostate cancer is located in 6q2 16q2 3,6q2 5 6q2 7 region. The genes coding for cyclin C and insulin-like growth factor II receptor are the candidate tumor suppressors in this region Genes, they may be related to the occurrence and development of prostate cancer.