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目的研究民族药方“扎里奴思方”对精神分裂症模型鼠海马组织中Cx43、谷氨酸及神经胶质细胞超微结构的影响。方法选择符合实验要求的实验大鼠60只,设置正常组,对照组(模型),实验组(ABC),每组12只大鼠,造模前,正常组和对照组予等量生理盐水灌胃,A、B、C组分别予40g/kg、20g/kg、10g/kg的等量药剂(扎里奴思方煎剂)灌胃,每天1次,连续给药21天,给药结束后2 h,各组(不包括正常组)予MK 801 0.6 mg/kg ip,建立实验动物模型,给药后3天处死动物,取海马组织用于检测。采用ABC-ELISA法检测Cx43含量,运用RT-PCR半定量法分析脑组织Cx43 mRNA表达情况,免疫组化检测海马组织中谷氨酸的表达,海马组织神经胶质细胞的超微结构用电镜进行观察。结果实验组(ABC)海马组织中的Cx43、Cx43 mRNA及谷氨酸活性表达均有不同程度升高,且减少了神经胶质细胞凋亡。结论《回回药方》中扎里奴思方对精神分裂症模型鼠海马组织中Cx43、谷氨酸及神经胶质细胞超微结构有积极影响,可能起到调节缝隙连接通讯作用。
Objective To investigate the effect of ethnic prescription “Zarimus prescription” on the ultrastructure of Cx43, glutamate and glial cells in hippocampus of schizophrenic model rats. Methods Sixty experimental rats were selected to meet the experimental requirements. Normal rats were divided into control group (model), experimental group (A B C), 12 rats in each group. Before modeling, normal group and control group The rats in groups A, B and C were given gavage with equal dosage of 40g / kg, 20g / kg and 10g / kg respectively (Zarimus decoction) once daily for continuous administration 21 days, 2 h after the administration, each group (excluding the normal group) was given MK 801 0.6 mg / kg ip to establish the experimental animal model. The animals were sacrificed 3 days after the administration, and the hippocampus tissues were taken for detection. The expression of Cx43 mRNA in hippocampus was detected by semi-quantitative RT-PCR. The expression of glutamate in hippocampus was detected by immunohistochemistry. The ultrastructure of hippocampus glial cells was observed by electron microscope . Results The expression of Cx43, Cx43 mRNA and glutamate in hippocampus of experiment group (A B C) increased to different extents and decreased the apoptosis of glial cells. Conclusion Zarimus prescription has a positive effect on the ultrastructure of Cx43, glutamate and glial cells in hippocampus of schizophrenia model rats, which may play a role in regulating the gap junctional communication.