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目的 研究体外成熟不同时间猪卵母细胞卵质膜的抗电击和电融合能力。 方法 利用电刺激 ,显微操作与电融合方法分别对体外成熟不同时间猪卵母细胞的抗电击和电融合能力进行了研究。 结果 1.培养48h卵母细胞质膜的抗电击能力显著高于培养 2 4h和 36 h的未成熟卵以及培养 6 0 h和 72 h的老化卵 ;2 .体外成熟48h的卵母细胞 ,在进行同卵或异卵核体移值时 ,其融合能力最强 (融合率 90 %以上 ) ;3.将小鼠 2、4、8细胞和桑葚期胚胎的卵裂球分别移入 IVM 48h去核猪卵母细胞 ,电融合率 (75 %～ 88.6 % )无统计学差异。 结论 同细胞核与细胞质成熟一样 ,细胞膜也存在成熟问题。培养 48h的猪卵母细胞质膜的抗电击能力和发生电融合的能力达到最强
Objective To study the anti-shock and electro-fusion ability of porcine oocyte oocyte membrane in vitro maturation. Methods The electrical shock resistance and electrofusion ability of porcine oocytes in vitro matured at different times were studied by electrical stimulation, micromanipulation and electrofusion respectively. Results 1. The shock resistance of plasma membrane of oocyte cultured for 48h was significantly higher than that of immature oocytes cultured for 24 h and 36 h and aged oocytes for 60 h and 72 h respectively.2. Oocytes matured in vitro for 48h, The fusion ability was the strongest (the fusion rate was more than 90%) when the same or the same oocyte was transferred. 3. The blastocysts of mouse 2,4,8 cells and mulberry embryo were transferred to IVM 48h Oocytes, electrical fusion rate (75% ~ 88.6%) was no significant difference. Conclusions As with the cell nucleus and cytoplasm maturation, there are also maturation problems in the cell membrane. The resistance of the plasma membrane of porcine oocyte cultured for 48 hours reached the strongest