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M12.4.1细胞(系小鼠B淋巴瘤细胞系),此细胞的倍增时间与培养中血清含量及植入细胞量有关,细胞以4.5×10~4/ml的浓度,植入含15%血清的培液中,其倍增时间为23.8h,M12.4.1-CM对小鼠骨髓CFU-mix的增殖有明显的加强作用,可使 CFU-mix数增加至2倍.单集落形态学分析结果表明,M12.4.1-CM可加强CFU-GEMm,CFU-GMm及p-BFU-E等早期造血祖细胞的增殖与分化.用亲和层析和高效液相色谱等技术,由M12.4.1细胞的无血清培养上清中,分离提纯到一种分子量为31000道尔顿的增殖放大因子,对CFU-mix增殖的活力比原液提高2809.2倍,从单集落分析结果表明,PAF调控早期造血祖细胞CFU-GEMm的活性非常明显地高于M12.4.1-CM.
M12.4.1 cells (a mouse B lymphoma cell line). The doubling time of this cell was related to the serum content in the culture and the amount of the implanted cells. The cells were implanted with 15% serum at a concentration of 4.5 × 10 4 / ml , The doubling time was 23.8h, and M12.4.1-CM enhanced the proliferation of mouse bone marrow CFU-mix significantly, which increased the number of CFU-mix to 2. The single colony morphological analysis showed that , M12.4.1-CM can enhance the proliferation and differentiation of early hematopoietic progenitor cells such as CFU-GEMm, CFU-GMm and p-BFU-E.M affinity chromatography and high performance liquid chromatography The supernatant of serum-free culture was isolated and purified to a proliferation magnification factor of 31000 daltons, which increased the viability of CFU-mix by 2809.2 times than that of the original solution. Analysis of single colonies showed that PAF regulates the proliferation of early hematopoietic progenitor cells CFU The activity of GMEM is significantly higher than that of M12.4.1-CM.