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目的:研究NF-κB在急性坏死性胰腺炎(ANP)相关性肺损伤中的表达改变情况及乌斯他丁的治疗作用机制。方法:采用逆行胰胆管牛磺胆酸钠(TAC)注射方法建立大鼠ANP肺损伤模型。SD大鼠分为假手术组(SO组),ANP生理盐水对照组(ANP组)和乌斯他丁治疗组。各组于术后3,6,12h剖杀,检验NF-κB的表达情况及肺组织髓过氧化物酶(MPO)活性和TNF-α的变化及肺组织的病理学改变。结果:相对于SO组,ANP组大鼠从3h开始,MPO及TNF-α增高,NF-κB表达于3h增高,6h达到高峰,给予乌斯他丁治疗后,肺损伤各指标均有不同程度降低(P<0.05),NF-κB表达明显降低(P<0.05)。结论:ANP相关肺损伤与NF-κB的激活有关,乌斯他丁通过下调NF-κB表达对ANP相关性肺损伤起保护作用。
Objective: To investigate the expression of NF-κB in acute necrotizing pancreatitis (ANP) -related lung injury and the mechanism of action of ulinastatin. Methods: The model of ANP-induced lung injury in rats was established by injecting sodium taurocholate (TAC) into the pancreatic duct. SD rats were divided into sham operation group (SO group), ANP saline control group (ANP group) and ulinastatin treatment group. The rats were killed at 3, 6 and 12 h after operation. The expression of NF-κB and the activity of MPO and TNF-α in lung tissue and the pathological changes of lung tissue were detected. RESULTS: Compared with SO group, the levels of MPO and TNF-α in the ANP group increased from 3h, the expression of NF-κB increased at 3h and peaked at 6h, and the indexes of lung injury in the ANP group were all different (P <0.05), and the expression of NF-κB decreased significantly (P <0.05). CONCLUSION: ANP-associated lung injury is associated with the activation of NF-κB. Ustatin exerts a protective effect on ANP-related lung injury by down-regulating the expression of NF-κB.