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目的：对ｐＧＳｊ２４克隆化基因进行核苷酸序列分析，了解其编码蛋白的属性。方法：常规制备ｐＧＳｊ２４克隆化基因并重组入测序载体Ｍ１３ｍｐ１９，以ＤＹＥＰＲＩＭＥＲ荧光测序试剂盒进行核苷酸序列测定。分别以ＤＮＡＳＩＳ和ＧＯＬＤＫＥＹ软件对序列资料进行分析。结果：ｐＧＳｊ２４克隆化基因长８４０ｂｐ，含一开放阅读框，可编码一分子量为２２．６ｋＤａ的蛋白质。开读框上游和下游均有终止密码子。该基因与已发表的日本血吸虫２２．６ｋＤａ蛋白的编码基因同源性达９５％，编码区同源性达９９．７％。在该基因内有一段典型的ＥＦ－Ｈａｎｄ钙结合区序列，并有内质网导肽、微体导向信号等功能位点。预测该蛋白质内可能的抗原决定簇位置为第２９－３２、６３－６８和８７－１０１等氨基酸片段。结论：ｐＧＳｊ２４克隆化基因为日本血吸虫２２．６ｋＤａ抗原编码基因。
OBJECTIVE: To analyze the nucleotide sequence of pGSj24 cloned gene and understand the properties of its encoded protein. Methods: The cloned gene of pGSj24 was routinely prepared and sequenced. Sequencing vector M13mp19 was sequenced and its nucleotide sequence was determined by DYEPRIMER fluorescent sequencing kit. Sequence data were analyzed using DNASIS and GOLDKEY software, respectively. Results: The pGSj24 cloned gene was 840 bp in length and contained an open reading frame encoding a protein with a molecular weight of 22.6 kDa. There are stop codons upstream and downstream of the open reading frame. This gene has a homology of 95% with the published 22.6kDa protein of Schistosoma japonicum and a homology of 99.7% in the coding region. Within this gene there is a typical EF-Hand calcium binding region sequence, and endoplasmic reticulum lead, micro-oriented signal and other functional sites. The possible epitopes within this protein are predicted to be the amino acid residues 29-32, 63-68 and 87-101. Conclusion: The cloned gene of pGSj24 is the gene encoding 22.6kDa antigen of Schistosoma japonicum.