论文部分内容阅读
目的:探寻半夏化感物质的主要来源、存在部位及其作用浓度。方法:采用生物测试的方法,分别研究块茎浸提液、根系分泌物及其不同极性萃取部位对白菜种子萌发和幼苗生长的化感效应。结果:半夏块茎浸提液对白菜种子萌发有化感抑制作用,当浓度为0.1 g/mL时,化感抑制作用最强,对发芽率和发芽势的化感指数分别为-0.11和-0.26。半夏块茎浸提液对白菜幼苗生长的影响表现为低浓度促进,高浓度抑制规律。半夏块茎浸提液不同极性萃取部位对白菜种子萌发和幼苗生长都具有化感效应。半夏根系分泌物溶液对白菜种子萌发呈现低浓度促进,高浓度抑制作用。当浓度为1.284×10~(-2)g/mL时,呈显著抑制作用,对发芽率和发芽势的化感效应指数分别达到-0.64和-0.87。半夏根系分泌物溶液对白菜幼苗生长呈抑制作用,且浓度越高,抑制作用越强。当浓度为1.284×10~(-2)g/mL时,化感抑制作用指数达-0.95。半夏根系分泌物不同极性萃取部位对白菜种子萌发和幼苗生长的化感效应因浓度不同而呈现促进或抑制作用。结论:半夏块茎和根系分泌物中含有能对白菜种子萌发和幼苗生长起化感作用的化感物质,既有弱极性的,也有中等极性的,还有强极性的。
Objective: To explore the main source of allelochemicals in Pinellia ternata, the existence sites and their concentration of action. Methods: The bioassay methods were used to study the allelopathic effects of extracts of tuberous roots, root exudates and their extracts with different polarities on seed germination and seedling growth of Chinese cabbage. Results: Pinellia ternata extract had allelopathic inhibitory effect on the germination of Chinese cabbage. When its concentration was 0.1 g / mL, the allelopathic inhibitory effect was the strongest. The allelochemical index of germination rate and germination potential were -0.11 and - 0.26. Pinellia tuber leaching solution on the growth of cabbage seedlings showed the role of low concentration, high concentration inhibition. Pinellia tuber leaching solution extracts of different polarities have a allelopathic effect on the seed germination and seedling growth of Chinese cabbage. Pinellia root secretion solution of cabbage seed germination showed a low concentration to promote the high concentration of inhibition. When the concentration was 1.284 × 10 ~ (-2) g / mL, the allelopathic effect indexes of germination and germination potentials were -0.64 and -0.87 respectively. Pinellia decoction secretion of Chinese cabbage seedling growth was inhibited, and the higher the concentration, the stronger the inhibitory effect. When the concentration was 1.284 × 10 ~ (-2) g / mL, the chemosensitive inhibition index reached -0.95. The allelopathic effect of different polarity extracts of Pinellia ternata on seed germination and seedling growth of cabbage was promoted or inhibited by different concentrations. CONCLUSION: The tuber and root exudates of Pinellia ternata contain allelochemicals that can sensitize cabbage to seed germination and seedling growth, both of which are weakly polar, moderately polar, and strongly polar.