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[目的]探讨hMSH2甲基化在食管癌发生发展中的作用。[方法]应用实时荧光甲基化特异性聚合酶链反应(real-time MSP)分别检测130例食管癌患者癌组织及癌旁正常组织中hMSH2甲基化情况,并分析其与临床病理等参数及预后之间的关系。[结果]在130例原发性食管癌组织中,hMSH2甲基化的有63例(48.5%),与之相对应的癌旁正常组织中甲基化的有18例(13.8%)(P<0.05)。hMSH2高甲基化分别与患者的年龄、肿瘤远处转移及不良预后有关(P<0.05),而与患者性别、淋巴结转移及临床分期等无关(P>0.05)。[结论]hMSH2高甲基化是食管癌频发的分子事件,也是导致其错配修复功能缺陷的重要原因之一。hMSH2基因启动子区甲基化可能在老龄化及食管癌的发生发展过程中发挥重要作用,是一个潜在的预后相关因子。
[Objective] To investigate the role of hMSH2 methylation in the development of esophageal cancer. [Methods] The methylation-specific methylation-specific polymerase chain reaction (real-time MSP) was used to detect the methylation status of hMSH2 in 130 cases of esophageal cancer and adjacent normal tissues respectively. And the relationship between the prognosis. [Results] There were 63 cases (48.5%) of hMSH2 methylation in 130 cases of primary esophageal cancer, and 18 cases (13.8%) of methylation in corresponding adjacent normal tissues (P <0.05). Hypermethylation of hMSH2 was associated with age, distant metastasis and poor prognosis (P <0.05), but not with gender, lymph node metastasis and clinical stage (P> 0.05). [Conclusion] Hypermethylation of hMSH2 is a frequent molecular event of esophageal cancer and is also one of the important reasons leading to its mismatch repair defect. Methylation of hMSH2 gene promoter may play an important role in the development of aging and esophageal cancer, which is a potential prognostic factor.